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cas9 gene coding sequence  (Epoch Life Signs Inc)
90
Epoch Life Signs Inc cas9 gene coding sequence
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Cas9 Gene Coding Sequence, supplied by Epoch Life Signs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 gene coding sequence/product/Epoch Life Signs Inc
Average 90 stars, based on 1 article reviews
cas9 gene coding sequence - by Bioz Stars, 2026-03
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cas9 coding sequence  (Taihe Biotechnology Co Ltd)
90
Taihe Biotechnology Co Ltd cas9 coding sequence
Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the <t>Cas9</t> gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.
Cas9 Coding Sequence, supplied by Taihe Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cas9 coding sequence/product/Taihe Biotechnology Co Ltd
Average 90 stars, based on 1 article reviews
cas9 coding sequence - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the Cas9 gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.

Journal: Scientific Reports

Article Title: Generation of Trichoderma harzianum with pyr4 auxotrophic marker by using the CRISPR/Cas9 system

doi: 10.1038/s41598-020-80186-4

Figure Lengend Snippet: Screening of T. harzianum mutants resistant to 5′-FOA. ( A ) Schematic representation of vectors used to transform fungus spores. ( B ) Genomic DNA of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ) was isolated and screened by PCR for the presence of the Cas9 gene which yielded a specific amplicon of 1242-bp. ( C ) Phenotype and growth of T. harzianum wild-type (WT) and FOA-resistant mutants ( ΔP3 , ΔP4 , ΔP7 , and ΔP13 ). *Bars marked with asterisk differ significantly ( P < 0.05). ( D ) A fragment from pyr4 , which was used for sequencing, confirmed mutants’ indels at target region. The sgRNA guiding sequence is highlighted in bold.

Article Snippet: For the construction of the CRISPR/Cas9 editing system, the Cas9 gene coding sequence from S. pyogenes was codon-optimized for expression in Trichoderma harzianum and synthesized by Epoch Life Science, Inc. (Sugar Land, TX, USA) ( ).

Techniques: Isolation, Amplification, Sequencing